Flow cytometric analysis of the reactivity of workshop monoclonal antibodies on sheep lymph node cells, after infection with Salmonella abortusovis

Vet Immunol Immunopathol. 1996 Aug;52(4):403-9. doi: 10.1016/0165-2427(96)05593-6.

Abstract

Of the 302 monoclonal antibodies included in the Third Workshop on Ruminant Leukocyte Antigens, 167 have been tested for their reactivity on uninfected and Salmonella-infected sheep lymph node leukocytes using FACS analysis. Only 47 of them showed specificities which could be related to those of some control monoclonal antibodies, such as the anti-CD5, anti-Ig light chain and anti-MHC Class II monoclonal antibodies.

MeSH terms

  • Animals
  • Antibodies, Bacterial / classification
  • Antibodies, Bacterial / immunology
  • Antibodies, Monoclonal / classification*
  • Antibodies, Monoclonal / immunology*
  • Antibody Specificity / immunology*
  • Flow Cytometry / methods*
  • Lymph Nodes / cytology*
  • Lymph Nodes / immunology*
  • Lymph Nodes / microbiology
  • Salmonella / classification
  • Salmonella / immunology*
  • Salmonella / pathogenicity*
  • Salmonella Infections, Animal / immunology*
  • Sheep

Substances

  • Antibodies, Bacterial
  • Antibodies, Monoclonal