Analysis of T cell receptor beta chains in the rat: I. Allelic polymorphism of V beta 8.2 is not a predisposing genetic factor in susceptibility to experimental allergic encephalomyelitis

J Neurosci Res. 1996 Sep 15;45(6):700-5. doi: 10.1002/(SICI)1097-4547(19960915)45:6<700::AID-JNR6>3.0.CO;2-C.

Abstract

Previous studies of experimental allergic encephalomyelitis (EAE) in the LER rat have suggested that amino acid differences present in the LER TCR V beta 8.2 chain may be associated with disease resistance. We report here that LEW rats bred to express a V beta 8.2 gene from DA rats, identical to that found in LER, are susceptible to EAE induction. Furthermore, T cells infiltrating the spinal cord of diseased animals primarily utilized V beta 8.2 and the associated AspSer CDR3 motif, typical of TCR V beta 8.2 chains expressed by pathogenic, anti-MBP responsive T cells in the LEW rat.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alleles
  • Animals
  • Encephalomyelitis, Autoimmune, Experimental / genetics*
  • Encephalomyelitis, Autoimmune, Experimental / metabolism
  • Gene Rearrangement, beta-Chain T-Cell Antigen Receptor
  • Genetic Predisposition to Disease
  • Haplotypes
  • Heterozygote
  • Homozygote
  • Lymph Nodes / immunology*
  • Male
  • Polymorphism, Genetic*
  • Rats
  • Rats, Inbred Lew
  • Rats, Inbred Strains
  • Receptors, Antigen, T-Cell, alpha-beta / chemistry
  • Receptors, Antigen, T-Cell, alpha-beta / genetics*
  • Spinal Cord / immunology
  • T-Lymphocytes / immunology*

Substances

  • Receptors, Antigen, T-Cell, alpha-beta