The sensitivity and specificity of intradermal tuberculin test (Tb test) were studied in an infected red deer (Cervus elaphus) herd (n = 109) in comparison to the results of other examinations including gross pathology, histopathology, Mycobacterium bovis culture, and the lymphocyte-stimulation test (LST). In our case, relative sensitivity was 79.5% and relative specificity was 45.7%. The predictive value in negative and positive cases was 80.0% and 44.9%, respectively. On the basis of the results obtained, eradication of tuberculosis by the selection procedure from a herd of newly captured and colonized deer was performed using the Tb test. For the purposes of eradication by selection all deer responding to B-PPD were considered positive.