Determination of 1-O-acyl-2-acetyl-sn-glyceryl-3-phosphorylcholine, platelet-activating factor and related phospholipids in biological samples by high-performance liquid chromatography--tandem mass spectrometry

J Chromatogr B Biomed Appl. 1996 Jun 28;682(1):35-45. doi: 10.1016/0378-4347(96)00070-9.

Abstract

Combining normal-phase HPLC separation and tandem mass spectrometric detection, using an ion-spray HPLC-MS interface, a quantitative method for acyl-platelet activating factor (acyl-PAF), platelet-activating factor (PAF) and related phospholipids was developed. Mass spectra, positive ions, showed intense [M+H]+ ions; collision-induced dissociation of protonated molecular ions gave characteristic daughter ions corresponding to the polar head. Detection limits of 0.1-0.3 ng injected were obtained by multiple reaction monitoring. Samples of human endothelial cells treated with compounds modulating the levels of acyl-PAF and PAF have been analyzed by the present technique, proving that this approach is suitable for biochemical studies.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cells, Cultured
  • Chromatography, High Pressure Liquid
  • Endothelium, Vascular / chemistry
  • Endothelium, Vascular / cytology
  • Humans
  • Linear Models
  • Lysophosphatidylcholines / analysis
  • Lysophosphatidylcholines / chemistry
  • Mass Spectrometry
  • Phospholipid Ethers / analysis
  • Phospholipid Ethers / chemistry
  • Phospholipids / analysis*
  • Phospholipids / chemistry
  • Platelet Activating Factor / analogs & derivatives*
  • Platelet Activating Factor / analysis*
  • Platelet Activating Factor / chemistry
  • Reference Standards
  • Umbilical Veins

Substances

  • 1-acyl-2-acetyl-sn-glycero-3-phosphocholine
  • Lysophosphatidylcholines
  • Phospholipid Ethers
  • Phospholipids
  • Platelet Activating Factor
  • CV 3988