Ultrastructural study of degradation of calcium phosphate ceramic by human monocytes and modulation of this activity by HILDA/LIF cytokine

J Histochem Cytochem. 1996 Oct;44(10):1131-40. doi: 10.1177/44.10.8813078.

Abstract

Biodegradation of ceramics in vivo is achieved essentially by monocytes and multinuclear cells (osteoclasts). Monocytes are the key element in this process because they intervene first at the biomaterial implantation site during inflammatory reaction. In this work, in vitro studies were conducted on an ultrastructural scale to determine the specific behavior of these cells with regard to a calcium phosphate (CaP) ceramic. Two types of phagocytosis were observed when cells came into contact with the biomaterial: either CaP crystals were taken up alone and then dissolved in the cytoplasm after disappearance of the phagosome membrane or they were incorporated together with large quantities of culture medium, in which case dissolution occurred after the formation of heterophagosomes. Phagocytosis of CaP coincided with autophagy and the accumulation of residual bodies in the cells. Addition of HILDA/LIF factor to these cultures induced a very marked decrease in phagocytotic activity directed at the capture of CaP crystals and culture medium. Autophagy was reduced, and residual bodies were rare or absent. This study specifies the role of monocytes in CaP biodegradation and demonstrates for the first time that HILDA/LIF has a biological effect on this cell line.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biodegradation, Environmental / drug effects
  • CHO Cells
  • Cell Differentiation / drug effects
  • Cells, Cultured
  • Ceramics / metabolism*
  • Cricetinae
  • Durapatite / metabolism*
  • Foreign-Body Reaction / metabolism
  • Foreign-Body Reaction / pathology*
  • Growth Inhibitors / pharmacology*
  • Humans
  • Interleukin-6*
  • Leukemia Inhibitory Factor
  • Lymphokines / pharmacology*
  • Macrophages / drug effects
  • Macrophages / metabolism
  • Macrophages / ultrastructure
  • Microscopy, Electron
  • Monocytes / drug effects
  • Monocytes / metabolism*
  • Monocytes / ultrastructure
  • Phagocytosis*
  • Phagosomes / metabolism
  • Phagosomes / ultrastructure
  • Recombinant Proteins / pharmacology

Substances

  • Growth Inhibitors
  • Interleukin-6
  • LIF protein, human
  • Leukemia Inhibitory Factor
  • Lymphokines
  • Recombinant Proteins
  • Durapatite