A procedure for the large-scale isolation of major bovine seminal plasma proteins

Protein Expr Purif. 1996 Aug;8(1):48-56. doi: 10.1006/prep.1996.0073.

Abstract

A method for the stepwise purification of bovine seminal plasma proteins aSFP, PDC-109, TIMP-2, RNAse, and BSP-30K/PDC-109 complex is presented. It is based on the use of nondenaturing, classical chromatographic procedures. The isolation protocol is reproducible, inexpensive, easy to carry out in a common laboratory, and yields large amounts of pure proteins for biochemical and structural studies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cattle
  • Chromatography, Affinity
  • Chromatography, Agarose
  • Chromatography, Gel
  • Chromatography, Ion Exchange
  • Electrophoresis, Polyacrylamide Gel
  • Molecular Sequence Data
  • Prostatic Secretory Proteins*
  • Proteins / chemistry
  • Proteins / isolation & purification*
  • Ribonucleases / isolation & purification
  • Semen / chemistry
  • Seminal Plasma Proteins
  • Tissue Inhibitor of Metalloproteinase-2

Substances

  • Prostatic Secretory Proteins
  • Proteins
  • Seminal Plasma Proteins
  • beta-microseminoprotein
  • Tissue Inhibitor of Metalloproteinase-2
  • Ribonucleases