The serum selenium (Se) concentrations of apparently healthy 20-40 year old blood donors from different parts of Switzerland were determined by electrothermal atomic absorption spectrometry (GFAAS). Application of a rhodium/magnesium matrix-modifier resulted in improved performance in comparison with a palladium modifier. The method was validated by hydride ICP-MS and quality-controlled by independent analysis using GFAAS with palladium as matrix-modifier; no bias was detected. The serum Se concentrations for male (n = 387) and female (n = 243) subjects fell into a normal distribution with mean values and standard deviations of 96.0 +/- 13.3 micrograms/L (1.22 +/- 0.17 mumol/L) and 87.9 +/- 14.4 micrograms/L (1.11 +/- 0.18 mumol/L), respectively. These values corresponded well to the formerly estimated mean daily intakes. Small but significant differences in mean serum Se concentrations were found between genders, ethnic groups as well as geographic regions, whereas age had no influence. The overall Se status of the Swiss population is assessed as adequate, somewhat higher than in the countries adjacent to Switzerland, but lower than in the U.S.A. or Finland. There is no evidence that the Se status of the population has changed over the past 10 years. However, it appears that some Swiss population groups may have a borderline Se status.