Enzymatic degradation of cell walls of apples and characterization of solubilized products

Int J Biol Macromol. 1995 Dec;17(6):337-40. doi: 10.1016/0141-8130(96)81842-3.

Abstract

Partially depectinated apple cell walls were digested by pectin lyase or endoglucanase or a combination. By combining these commercial enzymes, a higher yield of 22.2% of carbohydrate material was obtained compared with only 13.9% and 5.7%, respectively, when using them singly. Only small amounts of carbohydrates were extracted by buffer (0.8%). The solubilized extracts were fractionated using a combination of ion-exchange chromatography and gel filtration. The individual subfractions were analysed for neutral sugar and uronic acid content. The results indicated the existence of a synergistic effect between pectin lyase and endoglucanase based on the percentage of material extracted.

MeSH terms

  • Carbohydrates / analysis
  • Carbohydrates / isolation & purification*
  • Cell Wall / chemistry
  • Cell Wall / metabolism*
  • Cellulase / metabolism*
  • Chromatography, Gel
  • Chromatography, Ion Exchange
  • Drug Synergism
  • Fruit / metabolism*
  • Polysaccharide-Lyases / metabolism*
  • Solubility
  • Uronic Acids / analysis

Substances

  • Carbohydrates
  • Uronic Acids
  • Cellulase
  • Polysaccharide-Lyases
  • pectin lyase