Some preliminary results on the action of rhamnogalacturonase on rhamnogalacturonan oligosaccharides from beet pulp

Int J Biol Macromol. 1995 Dec;17(6):333-6. doi: 10.1016/0141-8130(96)81841-1.

Abstract

Sugar-beet pulp was saponified and then hydrolysed with 0.1 M HCl at 80 degrees C for 72 h, and a rhamnogalacturonan fraction was isolated by ion-exchange chromatography on AG 1X8 resin. Four individual oligomers, and a mixture of oligomers with higher degrees of polymerization, were obtained by chromatography on BioGel P-4. They all presented the alpha-D-GalAp-(1[-->2)-alpha-L-Rhap-(1-->4)-alpha-D-GalAp -(1]n-->2)-L-Rhap structure (with n > or = 2) The five fractions were submitted to hydrolysis with rhamnogalacturonase. The enzyme was active on oligomers with degrees of polymerization > or = 10, and gave as main products alpha-L-Rhap-(1-->4)-alpha-D-GalAp-(1-->2)-alpha-L-Rhap++ +(1-->4)-D-GalAp and alpha-D-GalAp-(1--2)-alpha-L-Rhap-(1-->4)-alpha-D-GalAp++ +-(1-->2)-alpha-L-Rhap- 1-->4)-D-GalAp.

MeSH terms

  • Carbohydrate Sequence
  • Chromatography, Gel
  • Chromatography, Ion Exchange
  • Fungal Proteins / metabolism*
  • Hydrolysis
  • Molecular Sequence Data
  • Oligosaccharides / metabolism
  • Pectins / metabolism*
  • Plants, Edible / metabolism
  • Polysaccharide-Lyases / metabolism*
  • Substrate Specificity

Substances

  • Fungal Proteins
  • Oligosaccharides
  • rhamnogalacturonan II
  • Pectins
  • Polysaccharide-Lyases
  • rhamnogalacturonase B