Discrepancies in osmolal gaps and calculated alcohol concentrations

Arch Pathol Lab Med. 1996 Jul;120(7):637-41.

Abstract

Objectives: To estimate unexplained osmolal gaps (UEOG) during ethanol ingestions after accounting for measured ethanol concentrations and to explore the causes of observed discrepancies. DESIGN AND SPECIMENS: A random convenience sample of serum specimens with a request to perform osmometry for the detection of alcohols was studied.

Main outcome measures: Serum concentrations of sodium, glucose, urea nitrogen, ethanol, osmolality, and ethanol were measured by two analytic methods to determine calculated osmolalities and osmolal gaps using two commonly used formulas.

Results: In 79 serum specimens submitted for osmometry, mean ethanol concentrations were 199 +/- 154 (SD) mg/dL; 15 specimens had no detectable ethanol. After regression of the calculated ethanol concentrations and the measured ethanol concentrations, the Dorwart and Chalmers formula showed a large constant bias of 45.217 +/- 6.414 (SE) mOsm/kg in predicting measured ethanol concentrations. The Smithline and Gardner formula was similarly as precise (r = .9786), but had a much smaller bias of -3.929 +/- 6.623 (SE) mOsm/kg. Actual mean differences between measured and calculated osmolalities (including ethanol osmols) gave an estimate of UEOG seen during ethanol ingestions. Using the less biased formula, the mean UEOG was 1.4 +/- 8.5 mOsm/kg, giving a 95% upper limit of the reference range of 18 mOsm/kg in this population with ethanol ingestions. Three significant outliers were identified and their UEOG could be explained by the presence of mannitol, isopropanol, or possibly a change in the assumed value for fractional water, normally a constant in these formulas for calculated osmolality. Other specific causes of osmolal gap and UEOG are discussed.

Conclusion: The potential range of osmolal gaps seen during ethanol ingestions after accounting for ethanol is demonstrated to be greater than in previous studies, which have included a lower proportion of ethanol-intoxicated subjects in their study sample.

MeSH terms

  • 1-Propanol / analysis
  • 1-Propanol / poisoning
  • Acetone / analysis
  • Adult
  • Alcoholism / blood
  • Alcoholism / diagnosis
  • Blood Chemical Analysis / methods
  • Blood Chemical Analysis / standards*
  • Chromatography / methods
  • Ethanol / blood*
  • Humans
  • Linear Models
  • Male
  • Mannitol / chemistry
  • Molecular Weight
  • Osmolar Concentration
  • Regression Analysis
  • Reproducibility of Results

Substances

  • Acetone
  • Ethanol
  • Mannitol
  • 1-Propanol