Abstract
The yeast PDR5 locus encodes a 160-kDa member of the ABC family of transport proteins. Strains bearing a deletion of this locus are drug hypersensitive. Resistant revertants arise when cells are plated on cycloheximide medium. About one-third of these are cross resistant to other agents, including oligomycin, fluconazole and sulfometuron methyl. Most of the revertants exhibit linkage to the PDR5 locus and map in three locations. Curiously, the multi-drug resistance is not due to a single mutation. Most of the revertants behave as though they contained several tightly linked resistance factors.
Publication types
-
Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
-
ATP-Binding Cassette Transporters / biosynthesis
-
ATP-Binding Cassette Transporters / genetics*
-
Antifungal Agents / pharmacology*
-
Crosses, Genetic
-
Cycloheximide / pharmacology
-
Drug Resistance, Multiple / genetics*
-
Fluconazole / pharmacology
-
Fungal Proteins / biosynthesis
-
Fungal Proteins / genetics
-
Genes, Fungal
-
Genetic Linkage
-
Genotype
-
Membrane Proteins / biosynthesis
-
Membrane Proteins / genetics*
-
Microbial Sensitivity Tests
-
Oligomycins / pharmacology
-
Recombination, Genetic
-
Saccharomyces cerevisiae / drug effects
-
Saccharomyces cerevisiae / genetics*
-
Saccharomyces cerevisiae Proteins*
-
Sulfonylurea Compounds / pharmacology
Substances
-
ATP-Binding Cassette Transporters
-
Antifungal Agents
-
Fungal Proteins
-
Membrane Proteins
-
Oligomycins
-
PDR5 protein, S cerevisiae
-
Saccharomyces cerevisiae Proteins
-
Sulfonylurea Compounds
-
Fluconazole
-
Cycloheximide
-
sulfometuron methyl