Sciatic nerve injury in the adult rat: comparison of effects on oligosaccharide, CGRP and GAP43 immunoreactivity in primary afferents following two types of trauma

J Neurocytol. 1996 Mar;25(3):219-31. doi: 10.1007/BF02284798.

Abstract

Using immunocytochemical and morphometric techniques, the localisation of three neuronal oligosaccharide antigens (two lactoseries and one globoseries oligosaccharide) were studied in the spinal cord and dorsal root ganglia of adult rats following unilateral crushing or transection of the sciatic nerve. The expression of CGRP and GAP43 was also studied for comparison. We found that following transection of the nerve the expression of lactoseries oligosaccharides and CGRP was permanently depressed, whilst that of the globoseries antigen (SSEA4) was unaffected. However following crush trauma and subsequent regeneration after 2 months, only the expression of one lactoseries antigen, LA4 remained significantly depressed. Our results suggest that different subsets of sensory neurons vary in the rate of reaction to injury and that one subset of neurons expressing a lactoseries oligosaccharide antigen is particularly susceptible to axotomy-induced changes. Furthermore neurons expressing the globoseries oligosaccharide antigen SSEA4 appear to be relatively unaffected by peripheral axotomy.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Afferent Pathways / chemistry
  • Animals
  • Antibodies, Monoclonal
  • Axons / physiology
  • Calcitonin Gene-Related Peptide / analysis
  • Calcitonin Gene-Related Peptide / immunology*
  • GAP-43 Protein
  • Ganglia, Spinal / cytology
  • Immunohistochemistry
  • Ligation
  • Membrane Glycoproteins / analysis
  • Membrane Glycoproteins / immunology*
  • Nerve Crush
  • Nerve Regeneration / physiology
  • Nerve Tissue Proteins / analysis
  • Nerve Tissue Proteins / immunology*
  • Neurofilament Proteins / analysis
  • Neurofilament Proteins / immunology
  • Oligosaccharides / analysis
  • Oligosaccharides / immunology*
  • Rats
  • Rats, Sprague-Dawley
  • Sciatic Nerve / chemistry
  • Sciatic Nerve / cytology
  • Sciatic Nerve / injuries*

Substances

  • Antibodies, Monoclonal
  • GAP-43 Protein
  • Membrane Glycoproteins
  • Nerve Tissue Proteins
  • Neurofilament Proteins
  • Oligosaccharides
  • Calcitonin Gene-Related Peptide