Expression of mRNA encoding neurotrophic factors and its regulation in a hybrid neuronal cell line

Biochem Mol Biol Int. 1996 Apr;38(4):763-72.

Abstract

In a cultured hybrid neuronal cell line (BIM) which was produced between human neuroblastoma cells (IMR32) and thymidine auxotrophs (B3T) of rat nerve-like cells (B103), the mRNAs encoding ciliary neurotrophic factor (CNTF) and neurotrophins were detected by the polymerase chain reaction method. The conditioned medium of BIM cells enhanced choline acetyltransferase (ChAT) activity in septal neurons and survival of ciliary ganglion neurons. The mRNA expression of CNTF and neurotrophins in BIM cells was differently regulated by the stimulation with cAMP, FGF and retinoic acid. These data suggest multiple regulation and collaboration of neurotrophic factors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Brain-Derived Neurotrophic Factor
  • Cell Line
  • Chick Embryo
  • Choline O-Acetyltransferase / metabolism
  • Ciliary Neurotrophic Factor
  • Cyclic AMP / pharmacology
  • Fibroblast Growth Factors / pharmacology
  • Humans
  • Hybrid Cells
  • Molecular Sequence Data
  • Nerve Growth Factors / biosynthesis*
  • Nerve Growth Factors / genetics
  • Nerve Tissue Proteins / biosynthesis*
  • Nerve Tissue Proteins / genetics
  • Neuroblastoma
  • Neurons / drug effects
  • Neurons / metabolism*
  • Polymerase Chain Reaction
  • RNA, Messenger / biosynthesis*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Rats
  • Rats, Wistar
  • Tretinoin / pharmacology
  • Tumor Cells, Cultured

Substances

  • Brain-Derived Neurotrophic Factor
  • Ciliary Neurotrophic Factor
  • Nerve Growth Factors
  • Nerve Tissue Proteins
  • RNA, Messenger
  • Tretinoin
  • Fibroblast Growth Factors
  • Cyclic AMP
  • Choline O-Acetyltransferase