Identification of a HeLa mRNA fraction which corrects the mitomycin C sensitivity of irs1 cells

Mutat Res. 1996 May 15;363(1):9-14. doi: 10.1016/0921-8777(95)00055-0.

Abstract

The hamster cell mutant irs1 is defective in its response to DNA lesions caused by a variety of mutagens, particularly cross-linking agents. These cells have been assigned to complementation group 2 of X-ray-sensitive mutants and the mutated gene is called XRCC2(X-ray repair cross complementing). We have identified, by microinjection, a human mRNA fraction which can transiently correct the sensitivity of these cells to cross-linking agents. This fraction contains mRNAs of 3.5 kb (+/- 0.25) including, therefore, the transcript of the XRCC2 gene.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Survival
  • Cricetinae
  • DNA Repair / genetics*
  • Dose-Response Relationship, Drug
  • Genetic Complementation Test
  • HeLa Cells
  • Humans
  • Microinjections
  • Mitomycin / pharmacology*
  • Mutagens / pharmacology
  • RNA, Messenger / isolation & purification*
  • Radiation Tolerance / genetics

Substances

  • Mutagens
  • RNA, Messenger
  • Mitomycin