Abstract
To obtain direct evidence that human natural killer cell (NK) inhibitory receptors physically interact with polymorphic determinants of major histocompatibility complex class I molecules, a receptor termed NK-associated transcript-2 has been produced as a soluble fusion protein with the human IgG1 Fc portion. This soluble receptor binds specifically to HLA-C alleles with serine 77 and asparagine 80 in cell adhesion assays.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alleles
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Amino Acid Sequence
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Animals
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Base Sequence
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Binding Sites / immunology
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Cell Line
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Cell Line, Transformed
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Female
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HLA-B Antigens / genetics
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HLA-C Antigens / genetics
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Histocompatibility Antigens Class I / genetics
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Histocompatibility Antigens Class I / metabolism*
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Humans
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Killer Cells, Natural / chemistry
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Killer Cells, Natural / metabolism*
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Male
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Mice
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Molecular Sequence Data
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Receptors, Immunologic / biosynthesis
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Receptors, Immunologic / metabolism*
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Receptors, KIR
Substances
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HLA-B Antigens
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HLA-C Antigens
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Histocompatibility Antigens Class I
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Receptors, Immunologic
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Receptors, KIR