Electrophoresis of concanavalin A receptors along embryonic muscle cell membrane

Nature. 1977 Feb 17;265(5595):602-5. doi: 10.1038/265602a0.

Abstract

Fluorescent concanavalin A (con A)-labelling showed that an electric field of 4 V cm-1 grossly redistributed con A receptors alone the plasma membranes of living muscle cells within 4 h. This field produced a voltage drop of 12 mV across these 30 micronm-wide cells. The movement of receptors was independent of cell metabolism and seemed to be electrophoretic in nature.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antimetabolites / pharmacology
  • Cell Membrane / analysis*
  • Cell Membrane / drug effects
  • Cells, Cultured
  • Electrophoresis
  • Embryo, Nonmammalian / ultrastructure
  • Fluoresceins
  • Microscopy, Fluorescence
  • Muscles / ultrastructure
  • Receptors, Concanavalin A / analysis*
  • Receptors, Drug / analysis*
  • Time Factors
  • Xenopus

Substances

  • Antimetabolites
  • Fluoresceins
  • Receptors, Concanavalin A
  • Receptors, Drug