Abstract
The yeast PDR1 locus encodes a member of the C6 zinc cluster family of transcriptional regulatory proteins. Among the targets of PDR1 is the yeast PDR5 locus. The product of this gene is a member of the ATP-binding cassette (ABC) transmembrane protein family and plays a major role in inhibitor efflux. Mutations in PDR1 affect the relative level of PDR5 transcript and can therefore result in increased or decreased drug resistance. We isolated three second-site suppressors of a PDR1-7 semidominant hyper-resistant mutation. These mutants were drug hypersensitive, as compared with isogenic controls. Two of the three mutations contained alterations in a putative DNA-binding domain. Significantly, the mutant proteins exhibited reduced DNA-binding capacity.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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ATP-Binding Cassette Transporters / genetics
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ATP-Binding Cassette Transporters / metabolism
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Alleles
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Base Sequence
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DNA Primers / chemistry
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism
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Drug Resistance, Multiple*
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Gene Expression Regulation, Fungal
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Genes, Suppressor
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Macromolecular Substances
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Molecular Sequence Data
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RNA, Messenger / genetics
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae Proteins
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Trans-Activators*
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Transcription Factors / genetics*
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Transcription, Genetic
Substances
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ATP-Binding Cassette Transporters
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DNA Primers
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DNA-Binding Proteins
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Macromolecular Substances
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PDR1 protein, S cerevisiae
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RNA, Messenger
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Saccharomyces cerevisiae Proteins
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Trans-Activators
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Transcription Factors