Isolation of enterotoxigenic Escherichia coli from British troops in Saudi Arabia

Epidemiol Infect. 1995 Dec;115(3):455-63. doi: 10.1017/s0950268800058611.

Abstract

Specimens from 181 patients with diarrhoea were examined by a Military General Hospital in a 3-month period during deployment of troops to Saudi Arabia in 1990/1. DNA probes for heat labile (LT) and heat stable (ST) enterotoxin genes identified enterotoxigenic Escherichia coli (ETEC) in 47 of the specimens (26%) and 49 ETEC strains were isolated. The majority (55%) belonged to a novel ETEC serotype having the O-antigen 159 and a flagellar antigen designated as a provisional new type. They produced ST and the coli surface associated antigen (CS)6. Strains of serotype O6:H16 represented 22% of the ETEC examined. They produced ST, LT and CS3 together with either CS1 or CS2. The remaining ETEC belonged to seven O:H serotypes. Overall, ST was the only enterotoxin gene identified in 73% of the ETEC and 67% of the strains expressed CS6 in the absence of other colonization antigens. Resistance to three or more antibiotics was observed in 53% of the ETEC, including most of the O159 strains.

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Antigens, Bacterial / analysis
  • Bacterial Proteins / analysis
  • Bacterial Toxins / biosynthesis*
  • DNA Probes
  • DNA, Bacterial / analysis
  • Diarrhea / epidemiology*
  • Diarrhea / microbiology
  • Drug Resistance, Microbial
  • Enterotoxins / biosynthesis*
  • Escherichia coli / classification
  • Escherichia coli / drug effects
  • Escherichia coli / isolation & purification*
  • Escherichia coli / metabolism
  • Escherichia coli Infections / epidemiology*
  • Escherichia coli Infections / microbiology
  • Escherichia coli Proteins*
  • Feces / microbiology
  • Humans
  • Microbial Sensitivity Tests
  • Military Personnel*
  • Rectum / microbiology
  • Saudi Arabia / epidemiology
  • Serotyping
  • United Kingdom / ethnology

Substances

  • Anti-Bacterial Agents
  • Antigens, Bacterial
  • Bacterial Proteins
  • Bacterial Toxins
  • DNA Probes
  • DNA, Bacterial
  • Enterotoxins
  • Escherichia coli Proteins
  • heat stable toxin (E coli)
  • heat-labile enterotoxin, E coli