Establishment of immortal hepatocytes from a CYP3A7-transgenic/p53-knockout mouse

M Shimoji; K Hattori; S Itoh; K Nakayama; M Katsuki; S Aizawa; T Yokoi; T Kamataki

doi:10.1006/bbrc.1995.2869

Establishment of immortal hepatocytes from a CYP3A7-transgenic/p53-knockout mouse

Biochem Biophys Res Commun. 1995 Dec 26;217(3):1001-5. doi: 10.1006/bbrc.1995.2869.

Authors

M Shimoji¹, K Hattori, S Itoh, K Nakayama, M Katsuki, S Aizawa, T Yokoi, T Kamataki

Affiliation

¹ Division of Drug Metabolism, Faculty of Pharmaceutical Sciences, Hokkaido University, Japan.

PMID: 8554550
DOI: 10.1006/bbrc.1995.2869

Abstract

Immortal hepatocytes were established from a CYP3A7-transgenic/p53-knockout mouse. The cells could be maintained with shorter doubling time after culture for 1 month. Detectable amounts of CYP3A7 mRNA and the activity of 7-propoxycoumarin O-depropylase, one of the representative CYP3A activities, were present in the immortal hepatocytes. Albumin mRNA, which is specifically expressed in the liver, remained in these cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

7-Alkoxycoumarin O-Dealkylase / genetics*
Animals
Cells, Cultured
Cytochrome P-450 Enzyme System / genetics*
Gene Expression Regulation, Developmental
Genes, p53
Humans
Liver / cytology*
Liver / enzymology
Mice
Mice, Knockout
Mice, Transgenic
RNA, Messenger / genetics

Substances

RNA, Messenger
Cytochrome P-450 Enzyme System
7-Alkoxycoumarin O-Dealkylase