Determination of Ras and GTPase-activating protein interactions by kinetic competition assay

Methods Enzymol. 1995:255:171-8. doi: 10.1016/s0076-6879(95)55021-6.
No abstract available

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Binding, Competitive
  • Escherichia coli / genetics
  • GTPase-Activating Proteins
  • Guanosine Triphosphate / metabolism
  • Hydrolysis
  • In Vitro Techniques
  • Kinetics
  • Molecular Sequence Data
  • Peptide Fragments / genetics
  • Peptide Fragments / metabolism
  • Peptide Fragments / pharmacology
  • Point Mutation
  • Protein Binding
  • Proteins / analysis
  • Proteins / metabolism*
  • Recombinant Proteins / genetics
  • Recombinant Proteins / isolation & purification
  • Recombinant Proteins / metabolism
  • ras GTPase-Activating Proteins
  • ras Proteins / genetics
  • ras Proteins / isolation & purification
  • ras Proteins / metabolism*

Substances

  • GTPase-Activating Proteins
  • Peptide Fragments
  • Proteins
  • Recombinant Proteins
  • ras GTPase-Activating Proteins
  • Guanosine Triphosphate
  • ras Proteins