Solution conformation of the tetrasaccharide glycoside Xyl beta 1-(Man alpha 1-3)2Man beta 1-4Glc beta 1R from the mollu-series glycosphingolipids

Biochem Biophys Res Commun. 1993 May 14;192(3):1057-65. doi: 10.1006/bbrc.1993.1524.

Abstract

The conformation of Xyl beta 1-(Man alpha 1-3)2Man beta 1-4Glc beta 1-OCH2CH(N3)CH(OH) CH = CHC13H27 tetrasaccharide glycoside in Me2SO-d6 was investigated with use of a distance mapping procedure based on rotating-frame NOE and hydrogen bond constraints, and Molecular Mechanics calculations. The Man alpha 1-3Man beta linkage was found to be well defined in a single conformation, whereas some flexibility could be assumed for the remaining two glycosidic linkages. Similar conclusions, though based on a smaller number of constraints, were drawn for the title compound anchored in mixed D2O/dodecylphosphocholine-d38 micelles.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbohydrate Conformation
  • Carbohydrate Sequence
  • Glycosphingolipids / chemistry*
  • Hydrogen Bonding
  • Magnetic Resonance Spectroscopy
  • Models, Molecular
  • Molecular Sequence Data
  • Molecular Structure
  • Oligosaccharides / chemistry*
  • Oligosaccharides / isolation & purification
  • Solutions

Substances

  • Glycosphingolipids
  • Oligosaccharides
  • Solutions