Method for sequential staining of GTL-banded metaphases with fluorescent-labeled chromosome-specific paint probes

Am J Med Genet. 1993 Apr 1;46(1):98-103. doi: 10.1002/ajmg.1320460117.

Abstract

We describe a method for use of fluorescent-labeled whole chromosome-specific paint probes on GTL-banded metaphases to utilize the combined potential of these techniques for defining chromosome abnormalities. The efficacy of this method was tested on 6 cases involving different chromosome abnormalities and various tissues, including blood, amniotic fluid, skin fibroblasts, and bone marrow.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Abnormalities, Multiple / genetics
  • Abortion, Spontaneous / genetics
  • Adult
  • Amniocentesis
  • Chromosome Banding / methods*
  • Female
  • Fluorescent Dyes
  • Fragile X Syndrome / genetics
  • Humans
  • Hydrocephalus / genetics
  • In Situ Hybridization, Fluorescence
  • Infant
  • Infant, Newborn
  • Karyotyping
  • Leukemia, Promyelocytic, Acute / genetics
  • Male
  • Metaphase
  • Middle Aged
  • Mosaicism
  • Pregnancy
  • Translocation, Genetic

Substances

  • Fluorescent Dyes