Purification and crystallization of human cyclin-dependent kinase 2

J Mol Biol. 1993 Apr 20;230(4):1317-9. doi: 10.1006/jmbi.1993.1248.

Abstract

The major transitions of the eukaryotic cell cycle are triggered by cyclin-dependent protein kinases. We report the purification and crystallization of the catalytic subunit of human cyclin-dependent kinase 2 (CDK2), which has been implicated in the control of the G1/S transition. CDK2 was purified in large quantities from insect cells infected with a recombinant baculovirus, and crystals of the protein were prepared and subjected to preliminary X-ray diffraction analysis.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Baculoviridae / genetics
  • Base Sequence
  • CDC2-CDC28 Kinases*
  • Cells, Cultured
  • Crystallization
  • Cyclin-Dependent Kinase 2
  • Cyclin-Dependent Kinases*
  • Humans
  • Molecular Sequence Data
  • Moths
  • Protein Kinases / biosynthesis
  • Protein Kinases / genetics
  • Protein Kinases / isolation & purification*
  • Protein Serine-Threonine Kinases*
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / isolation & purification

Substances

  • Recombinant Proteins
  • Protein Kinases
  • Protein Serine-Threonine Kinases
  • CDC2-CDC28 Kinases
  • CDK2 protein, human
  • Cyclin-Dependent Kinase 2
  • Cyclin-Dependent Kinases