The large cytoplasmic domain is not required for concentration of N-CAM at cell-cell contacts in transfected mouse neuroblastoma cells

Exp Cell Res. 1993 Apr;205(2):331-6. doi: 10.1006/excr.1993.1094.

Abstract

We examined the localization of the 140- and 180-kDa transmembrane isoforms of chicken N-CAM following transfection into mouse N2A neuroblastoma cells. Both isoforms were expressed at the cell surface and became partially or completely localized at areas of cell-cell contact after several days of culture or of in vitro differentiation. These results indicate that the presence of the large cytoplasmic domain of the 180-kDa N-CAM isoform is not necessary to bring about the localization of N-CAM to points of cell-cell contact.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Adhesion Molecules, Neuronal / chemistry*
  • Cell Adhesion Molecules, Neuronal / metabolism
  • Cell Adhesion*
  • Chickens
  • Cytoplasm / ultrastructure
  • Fluorescent Antibody Technique
  • In Vitro Techniques
  • Mice
  • Neuroblastoma
  • Structure-Activity Relationship
  • Transfection
  • Tumor Cells, Cultured

Substances

  • Cell Adhesion Molecules, Neuronal