Abstract
Eukaryotic translation initiation factor 5 (eIF-5) catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.AUG.Met-tRNAf-eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the formation of a functional 80S initiation complex. A rat cDNA that encodes eIF-5 has been isolated and expressed in Escherichia coli to yield a catalytically active eIF-5 protein. The 3.55-kb cDNA encodes a protein of 429 amino acids (calculated M(r) 48,926) with properties that are similar to eIF-5 isolated from rabbit reticulocyte lysates. The deduced amino acid sequence of eIF-5 contains sequence motifs characteristic of proteins of the GTPase superfamily.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Cloning, Molecular / methods
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DNA / genetics*
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Escherichia coli / genetics
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Eukaryotic Initiation Factor-5
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GTP Phosphohydrolases / genetics
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Guanosine Triphosphate / metabolism
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Liver / physiology
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Macromolecular Substances
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Molecular Sequence Data
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Multigene Family
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Oligodeoxyribonucleotides
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Peptide Initiation Factors / genetics*
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Peptide Initiation Factors / isolation & purification
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Peptide Initiation Factors / metabolism
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Protein Biosynthesis
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RNA, Transfer, Amino Acyl / metabolism
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RNA, Transfer, Met*
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Rabbits
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Restriction Mapping
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Reticulocytes / metabolism
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Ribosomes / metabolism
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Sequence Homology, Amino Acid
Substances
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Eukaryotic Initiation Factor-5
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Macromolecular Substances
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Oligodeoxyribonucleotides
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Peptide Initiation Factors
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RNA, Transfer, Amino Acyl
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RNA, Transfer, Met
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tRNA, formylmethionine-
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Guanosine Triphosphate
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DNA
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GTP Phosphohydrolases