A rapid quantitative method for detecting infectious bursal disease virus using polystyrene latex microspheres

J Virol Methods. 1993 Jun;43(1):123-9. doi: 10.1016/0166-0934(93)90095-9.

Abstract

A monoclonal antibody (mAb) to infectious bursal disease virus (IBDV) was bound to polystyrene latex microspheres. The microspheres agglutinated with extracts of bursae and sera from chickens infected with all strains or isolates of IBDV tested. Agglutination appeared within a 10-min reaction time. The assay could detect a 10(3.7) to 10(4.5) mean embryo infective dose (EID50) of the virus in 0.01 ml and the titer of the assay was 10- to 40-times higher than that of the agar gel precipitin test.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology*
  • Antibodies, Viral / immunology*
  • Antigens, Viral / analysis
  • Bursa of Fabricius / microbiology
  • Chick Embryo / microbiology
  • Chickens / blood
  • Chickens / immunology
  • Chickens / microbiology*
  • Infectious bursal disease virus / immunology
  • Infectious bursal disease virus / isolation & purification*
  • Infectious bursal disease virus / pathogenicity
  • Latex Fixation Tests*
  • Mice
  • Mice, Inbred BALB C / immunology
  • Microspheres
  • Poultry Diseases / blood
  • Poultry Diseases / immunology
  • Poultry Diseases / microbiology*
  • Precipitin Tests
  • Reoviridae Infections / blood
  • Reoviridae Infections / immunology
  • Reoviridae Infections / microbiology
  • Reoviridae Infections / veterinary*
  • Sensitivity and Specificity
  • Time Factors
  • Viral Vaccines
  • Viremia / microbiology
  • Viremia / veterinary

Substances

  • Antibodies, Monoclonal
  • Antibodies, Viral
  • Antigens, Viral
  • Viral Vaccines