Sections from 88 paraffin-embedded tissues identified histopathologically as condyloma acuminata were assayed for the presence of human papillomavirus (HPV) types 6, 11, 16, 18 and 33 using polymerase chain reaction followed by specific hybridization. HPV DNA sequences were found in all tissues of condyloma acuminata. HPV type 6 was present in 71 tissues, type 11 in 16, and type 16 in 2. One man was doubly infected with HPV types 6 and 16. No HPV types 18 and 33 were found. We conclude that condyloma acuminata is mainly produced by HPV types 6 and 11.