Rapid detection of human group C rotaviruses by reverse passive hemagglutination and latex agglutination tests using monoclonal antibodies

J Clin Microbiol. 1993 May;31(5):1308-11. doi: 10.1128/jcm.31.5.1308-1311.1993.

Abstract

Reverse passive hemagglutination (RPHA) tests and a latex agglutination test with monoclonal antibodies (MAbs) were developed for the rapid detection of noncultivatable human group C rotaviruses. For RPHA tests, two MAbs, MAb 5A12 recognizing the outer capsid and MAb 13A3 recognizing the inner capsid, were separately used for the coating of sheep erythrocytes (SRBCs). Forty-six fecal samples were examined to confirm the practicality of the tests. As a result, there was concordance between the RPHA test with SRBCs coated with MAb 5A12 and polyacrylamide gel electrophoresis of viral RNA (RNA-PAGE) in 44 (95.6%) of 46 samples, while the diagnoses by the RPHA test with SRBCs coated with MAb 13A3 were in complete agreement with those by RNA-PAGE. Furthermore, a latex agglutination test with MAb 13A3 was also developed, and this test was fast enough and sensitive enough to successfully detect the viruses from most fecal samples within 2 min. The present procedures would be useful for the diagnosis of human group C rotavirus infections in clinical laboratories which are not well equipped.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal
  • Child, Preschool
  • Evaluation Studies as Topic
  • Gastroenteritis / diagnosis
  • Hemagglutination Tests / methods*
  • Hemagglutination Tests / statistics & numerical data
  • Humans
  • Infant
  • Latex Fixation Tests / methods*
  • Latex Fixation Tests / statistics & numerical data
  • Rotavirus / classification
  • Rotavirus / immunology
  • Rotavirus / isolation & purification*
  • Rotavirus Infections / diagnosis
  • Sensitivity and Specificity
  • Virology / methods
  • Virology / statistics & numerical data

Substances

  • Antibodies, Monoclonal