The effect of mannitol and nimodipine treatment in a rat model of temporary focal ischemia

Zentralbl Neurochir. 1993;54(1):3-12.

Abstract

Male Sprague Dawley rats were anesthetised with Xylazine and Ketamine intraperitoneally. After a lateral craniotomy the cerebral inferior vein was ligated and a very small clip (Biemer clip) was placed on the MCA near its origin for 1 hour. This procedure induced a focal infarction in 100% of the rats. After removal of the clip the lumen of the MCA was patent. The study was divided in 3 randomized groups (control group n = 15; Nimodipine group n = 11, treatment 30 micrograms/hour/kg body-weight; Mannitol group n = 15, treatment 5.4 ml/hour/kg body-weight). Besides heart-rate, ECG and blood pressure we measured the extracellular potassium and calcium concentration with ion-selective microelectrodes; the ICBF was estimated by laser-doppler-flowmeter. The MCA was clipped for 1 hour. After 1 hour of reperfusion the brain was fixated and the volume of infarction was measured by serial slices. Nimodipine or Mannitol treatment started 5 min before clipping the MCA. In rats with Nimodipine treatment the extracellular calcium starts at a significantly higher level (2.3 +/- 0.5 mmol/l) and the decrease during ischemia remains above a level of 1.2 +/- 0.2 mmol/l. The increase in potassium during ischemia and Nimodipine (calculated in change of concentration/time [dc/dt]) is significantly slower than in the control group. In contrast to the post-ischemic hyper- and hypoperfusion in the control group the reperfusion in the Mannitol group is nearly normal. In the control group the infarction volume is 20% of the brain, in the Nimodipine group 15% and in the Mannitol group only 11%. The calcium antagonist Nimodipine and the free-radical scavenger Mannitol therefore promise to be a way to treat or prevent temporary focal cerebral ischemia.

MeSH terms

  • Animals
  • Brain / blood supply
  • Brain Ischemia / pathology*
  • Calcium / metabolism
  • Free Radicals
  • Infusions, Intravenous
  • Male
  • Mannitol / pharmacology*
  • Myocardial Infarction / pathology*
  • Nimodipine / pharmacology*
  • Potassium / metabolism
  • Rats
  • Rats, Sprague-Dawley
  • Regional Blood Flow / drug effects
  • Superoxides / metabolism

Substances

  • Free Radicals
  • Superoxides
  • Mannitol
  • Nimodipine
  • Potassium
  • Calcium