Insight into topological and functional relationships of cytochrome c oxidase subunit I of Saccharomyces cerevisiae by means of intragenic complementation

B Meunier; F Coster; P Lemarre; A M Colson

doi:10.1016/0014-5793(93)80099-g

Insight into topological and functional relationships of cytochrome c oxidase subunit I of Saccharomyces cerevisiae by means of intragenic complementation

FEBS Lett. 1993 Apr 26;321(2-3):159-62. doi: 10.1016/0014-5793(93)80099-g.

Authors

B Meunier¹, F Coster, P Lemarre, A M Colson

Affiliation

¹ Université Catholique de Louvain, Laboratoire de Génétique Microbienne, Louvain-la-Neuve, Belgium.

PMID: 8386676
DOI: 10.1016/0014-5793(93)80099-g

Abstract

In yeast, revertants were selected from four respiratory deficient mutants carrying mutations in the cytochrome c oxidase subunit I gene. Intragenic second site mutations revealed amino acids which are functionally complementary to the original mutated position and may be in topological interaction with it. The results provide additional data in favour of the model proposed for the structure of the binuclear centre in proton-motive oxidases.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Base Sequence
Codon / genetics
Electron Transport Complex IV / chemistry*
Electron Transport Complex IV / metabolism*
Genes, Fungal
Genetic Complementation Test
Introns
Macromolecular Substances
Models, Structural
Molecular Sequence Data
Mutagenesis, Site-Directed
Mutation
Protein Structure, Secondary
Saccharomyces cerevisiae / enzymology*
Saccharomyces cerevisiae / genetics

Substances

Codon
Macromolecular Substances
Electron Transport Complex IV