Modified purification of L-aromatic amino acid decarboxylase from pig kidney

P Dominici; P S Moore; C B Voltattorni

doi:10.1006/prep.1993.1045

Modified purification of L-aromatic amino acid decarboxylase from pig kidney

Protein Expr Purif. 1993 Aug;4(4):345-7. doi: 10.1006/prep.1993.1045.

Authors

P Dominici¹, P S Moore, C B Voltattorni

Affiliation

¹ Università Cattolica del Sacro Cuore, Facoltà di Medicina e Chirurgia A. Gemelli, Istituto di Chimica Biologica, Rome, Italy.

PMID: 8374304
DOI: 10.1006/prep.1993.1045

Abstract

A new protocol for purification of L-aromatic amino acid decarboxylase from pig kidney in which, after an initial ultracentrifugation, only two columns are required to obtain homogeneous enzyme is presented. The protocol provides protein with a higher specific activity than that previously obtained, reducing by about one-half the time required for purification.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Aromatic-L-Amino-Acid Decarboxylases / chemistry
Aromatic-L-Amino-Acid Decarboxylases / isolation & purification*
Chromatography, Agarose
Kidney / enzymology*
Molecular Weight
Pyridoxal Phosphate
Sepharose / analogs & derivatives
Swine

Substances

Pyridoxal Phosphate
phenyl-sepharose
Sepharose
Aromatic-L-Amino-Acid Decarboxylases