Abstract
One of the monocytic cell colony stimulating factors produced by thymic myoid cells, a 100 kDa factor, was purified by reversed phase HPLC and found to be homologous to the secreted form of proteoglycan 1 (biglycan) core protein. This biglycan associated colony stimulating factor did not carry an immunological motif of macrophage colony stimulating factor (M-CSF), but predominantly stimulated the proliferation and differentiation of monocytic lineage cells from bone marrow cells, nonadherent thymic cells and peritoneal exudate cells.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Biglycan
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Bone Marrow
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Cell Differentiation / drug effects*
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Cell Division / drug effects*
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Cell Line
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Chromatography, High Pressure Liquid
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Chromatography, Ion Exchange
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Colony-Stimulating Factors / biosynthesis
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Colony-Stimulating Factors / isolation & purification
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Colony-Stimulating Factors / pharmacology*
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Extracellular Matrix Proteins
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Hematopoietic Stem Cells / cytology*
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Hematopoietic Stem Cells / drug effects
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Humans
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Macrophage Colony-Stimulating Factor / pharmacology
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Molecular Sequence Data
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Proteoglycans / biosynthesis
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Proteoglycans / isolation & purification
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Proteoglycans / pharmacology*
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Rats
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Recombinant Proteins / pharmacology
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T-Lymphocytes / cytology
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T-Lymphocytes / drug effects
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Thymus Gland / cytology
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Thymus Gland / drug effects
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Thymus Gland / metabolism*
Substances
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BGN protein, human
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Biglycan
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Colony-Stimulating Factors
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Extracellular Matrix Proteins
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Proteoglycans
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Recombinant Proteins
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biglycan associated colony-stimulating factor
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Macrophage Colony-Stimulating Factor