Use of the CTL44 cell line, a derivative of CTL/L cells, to identify and quantify mouse interleukin-4 by bioassay

J Immunol Methods. 1993 Sep 15;164(2):213-20. doi: 10.1016/0022-1759(93)90314-w.

Abstract

The isolation and characterization of a new and excellent indicator cell line for murine interleukin-4 (IL-4) bioassays, CTL44, is described. CTL44, a subline of CTL/L cells, is vigorously responsive to murine IL-4, but hyporesponsive to IL-2, requiring > 6 ng/ml (approximately 120 U/ml) of human IL-2 or > 80 U/ml of mouse IL-2 to induce IL-2 dependent proliferation. In CTL44 both IL-4 receptor mRNA accumulation and cell surface expression are detected, whereas IL-2 receptor expression appears to be absent. CTL44 cells maintained in IL-4 containing medium grow rapidly in a non-adherent fashion, and can be stored frozen in liquid nitrogen without loss of function.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biological Assay / methods
  • Cell Line
  • Gene Expression
  • Interleukin-4 / analysis*
  • Interleukins / pharmacology
  • Lymphocyte Activation
  • Mice
  • RNA, Messenger / genetics
  • Receptors, Interleukin-2 / genetics
  • Receptors, Interleukin-2 / metabolism
  • Receptors, Interleukin-4
  • Receptors, Mitogen / genetics
  • Receptors, Mitogen / metabolism
  • T-Lymphocytes, Cytotoxic / immunology*

Substances

  • Interleukins
  • RNA, Messenger
  • Receptors, Interleukin-2
  • Receptors, Interleukin-4
  • Receptors, Mitogen
  • Interleukin-4