Cloning and nucleotide sequence analysis of the Candida albicans enolase gene

Abstract

The complete nucleotide sequence of the coding region as well as the flanking non-coding region of Candida albicans enolase gene was determined. A continuous open reading frame of 1323 nucleotides with no introns was identified. The deduced amino acid sequence showed 87% similarity to the enolases from the yeast Saccharomyces cerevisiae. The two isoforms of enolase are encoded by two non-tandemly arrayed genes in S. cerevisiae. However, DNA hybridisation analysis indicates that in C. albicans enolase is encoded by a single gene. The position of the transcription start site, putative TATA box and polyadenylation signal of the C. albicans enolase gene have been identified. The location of these sequences are similar to those of the S. cerevisiae enolase genes.