Abstract
Using a murine monoclonal antibody, we were able to differentiate between mouse-virulent and -avirulent strains of Toxoplasma gondii. Monoclonal antibody TB6G5 was reactive with eight clinical mouse-avirulent isolates but not with mouse-virulent laboratory strains RH and BK. The antibody-reactive antigen was identified by indirect immunofluorescence and immunoblot as a 27-kDa cytoplasmic protein expressed by tachyzoites as well as by bradyzoites.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Monoclonal*
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Antigens, Protozoan / chemistry
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Cross Reactions
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Female
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Humans
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Mice
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Mice, Inbred BALB C
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Molecular Weight
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Species Specificity
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Toxoplasma / classification
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Toxoplasma / immunology*
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Toxoplasma / pathogenicity*
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Toxoplasmosis / immunology
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Toxoplasmosis, Animal / immunology
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Toxoplasmosis, Animal / microbiology
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Virulence / immunology
Substances
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Antibodies, Monoclonal
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Antigens, Protozoan