Insect calcium channels. Molecular cloning of an alpha 1-subunit from housefly (Musca domestica) muscle

FEBS Lett. 1994 Feb 14;339(1-2):189-94. doi: 10.1016/0014-5793(94)80413-3.

Abstract

The complete amino acid sequence of an invertebrate calcium channel alpha 1-subunit from housefly (Musca domestica) larvae (designated Mdl alpha 1) has been deduced by cDNA cloning and sequence analysis. Mdl alpha 1 shares higher percent sequence identity with 1,4-dihydropyridine (DHP)-sensitive L-type than with DHP-insensitive calcium channels. As shown by whole mount in situ hybridization and immunostaining Mdl alpha 1 is predominantly expressed in the larval body wall musculature.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Calcium Channels / genetics*
  • Cloning, Molecular*
  • DNA, Complementary / chemistry
  • DNA, Complementary / genetics
  • Dihydropyridines / pharmacology
  • Gene Expression
  • Houseflies / chemistry*
  • Houseflies / genetics
  • In Situ Hybridization
  • Larva / metabolism
  • Molecular Sequence Data
  • Muscles / metabolism
  • RNA, Messenger / analysis
  • RNA, Messenger / metabolism
  • Sequence Analysis
  • Sequence Homology
  • Tissue Distribution

Substances

  • Calcium Channels
  • DNA, Complementary
  • Dihydropyridines
  • RNA, Messenger
  • 1,4-dihydropyridine

Associated data

  • GENBANK/Z31723