Abstract
We developed a new method for detection of Candida albicans cells in human blood by the polymerase chain reaction that amplifies a 125-bp region within a species-specific, multiple-copy DNA fragment. In preparing template DNA from C. albicans-containing blood, the addition of C. tropicalis cells, together with antibody which reacts with both cells, raised the sensitivity of detection of C. albicans to about 3 cells per 0.1 ml.
MeSH terms
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Base Sequence
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Candida albicans / genetics*
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Candida albicans / isolation & purification*
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Candidiasis / diagnosis*
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Candidiasis / microbiology
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DNA Primers / genetics
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DNA, Fungal / genetics
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Evaluation Studies as Topic
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Fungemia / diagnosis*
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Fungemia / microbiology
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Humans
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Molecular Sequence Data
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Mycology / methods
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Mycology / statistics & numerical data
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Polymerase Chain Reaction / methods*
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Polymerase Chain Reaction / statistics & numerical data
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Sensitivity and Specificity