Abstract
The mannitol operon of Escherichia coli, encoding the mannitol-specific enzyme II of the phosphotransferase system (Mt1A) and mannitol phosphate dehydrogenase (Mt1D), is here shown to contain a single additional downstream open reading frame which encodes the mannitol repressor (Mt1R). Mt1R contains 195 amino acids and has a calculated molecular weight of 21,990 and a calculated pI of 4.5. It is homologous to the product of an open reading frame (URF2D) upstream of the E. coli gapB gene but represents a novel type of transcriptional regulatory protein.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Cloning, Molecular
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DNA Primers / chemistry
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Escherichia coli / genetics*
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Escherichia coli Proteins*
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Gene Expression Regulation, Bacterial
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Genes, Bacterial*
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Mannitol / metabolism
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Molecular Sequence Data
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Mutagenesis, Insertional
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Operon
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RNA, Messenger / genetics
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Repressor Proteins / genetics*
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Sequence Alignment
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Sequence Homology, Amino Acid
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Transcription, Genetic
Substances
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DNA Primers
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Escherichia coli Proteins
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MtlR protein, E coli
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RNA, Messenger
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Repressor Proteins
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Mannitol