Abstract
Conditioned media (CM) from various marrow subpopulations (MBA series) were examined for their effects on growth and maturation of marrow-derived osteoblastic cells, MBA-15. CM harvested from a stromal adipocytes, 14F1.1, stimulated cell growth, collagen but not non-collagenous proteins synthesized by MBA-15 cells. Alkaline phosphatase activity was inhibited under the same culture conditions. These results were associated with morphological changes, i.e., the large cuboidal MBA-15 cells acquired a fibroblast-like appearance. Exposure of MBA-15 cells to known growth factors: BMP-2, TGF beta, IGF-I and PDGF or combined with 14F1.1 CM resulted in a dominant effect of the latter. This may imply that marrow adipocytes produce factor/s that participate in the stromal regulation of osteoblastic functions.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Adipocytes / cytology
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Adipocytes / physiology*
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Alkaline Phosphatase / metabolism
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Bone Marrow / physiology*
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Bone Morphogenetic Proteins
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Cell Differentiation / drug effects
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Cell Division / drug effects
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Cell Line
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Collagen / biosynthesis
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Culture Media, Conditioned
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Growth Substances / pharmacology
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Humans
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Insulin-Like Growth Factor I / pharmacology
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Osteoblasts / cytology*
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Osteoblasts / drug effects
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Osteoblasts / metabolism
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Platelet-Derived Growth Factor / pharmacology
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Protein Biosynthesis
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Proteins / pharmacology
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Recombinant Proteins / pharmacology
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Transforming Growth Factor beta / pharmacology
Substances
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Bone Morphogenetic Proteins
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Culture Media, Conditioned
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Growth Substances
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Platelet-Derived Growth Factor
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Proteins
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Recombinant Proteins
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Transforming Growth Factor beta
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Insulin-Like Growth Factor I
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Collagen
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Alkaline Phosphatase