Strings of contiguous modified pentanucleotides with increased DNA-binding affinity can be used for DNA sequencing by primer walking

T Azhikina; S Veselovskaya; V Myasnikov; V Potapov; O Ermolayeva; E Sverdlov

doi:10.1073/pnas.90.24.11460

Strings of contiguous modified pentanucleotides with increased DNA-binding affinity can be used for DNA sequencing by primer walking

Proc Natl Acad Sci U S A. 1993 Dec 15;90(24):11460-2. doi: 10.1073/pnas.90.24.11460.

Authors

T Azhikina¹, S Veselovskaya, V Myasnikov, V Potapov, O Ermolayeva, E Sverdlov

Affiliation

¹ Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow.

Abstract

Modified oligonucleotides containing 5-methylcytidine and/or 2-aminoadenosine form tighter hybrids with DNA and are, therefore, more efficient primers for DNA sequencing as compared to their natural counterparts. Strings of contiguous modified pentanucleotides can be used for DNA sequencing by primer walking.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Adenosine / analogs & derivatives
Amides
Base Sequence
Cytidine / analogs & derivatives
DNA / chemistry*
DNA Primers / chemical synthesis
Indicators and Reagents
Molecular Sequence Data
Oligodeoxyribonucleotides / chemical synthesis
Oligodeoxyribonucleotides / chemistry*
Phosphoric Acids
Structure-Activity Relationship

Substances

Amides
DNA Primers
Indicators and Reagents
Oligodeoxyribonucleotides
Phosphoric Acids
2-aminoadenosine
Cytidine
DNA
phosphoramidic acid
Adenosine
5-methylcytidine