Cloning of VH and VL genes for antibodies specific for pyrimidine photo dimers

Nucleic Acids Symp Ser. 1993:(29):111-2.

Abstract

We have prepared two types of cDNAs encoding mouse immunoglobulin heavy (VH) and light (VL) chain variable (V) domains against UV-induced DNA damage by the polymerase chain reaction (PCR). The amplified cDNA was cloned for sequencing and expression, and the nucleotide sequences of the V domains were determined. The deduced amino acid sequences of the complementarity-determining regions (CDRs) indicated that CDR3 of the VH domain and CDR2 of the VL domain might be concerned with the recognition of photolesions.

MeSH terms

  • Antibodies / genetics*
  • Antibodies / immunology
  • Cloning, Molecular
  • DNA, Complementary
  • Hybridomas
  • Immunoglobulin Heavy Chains / genetics*
  • Immunoglobulin Light Chains / genetics*
  • Polymerase Chain Reaction
  • Pyrimidine Dimers / immunology*
  • Ultraviolet Rays

Substances

  • Antibodies
  • DNA, Complementary
  • Immunoglobulin Heavy Chains
  • Immunoglobulin Light Chains
  • Pyrimidine Dimers