Abstract
Twenty sheep were divided into groups and inoculated by various routes with recombinant raccoon poxvirus expressing the CVS rabies virus glycoprotein (rRCNV-G) or with raccoon poxvirus (RCNV). The apparent innocuous pathologic responses to each virus coupled with development of high levels of rabies virus neutralizing antibodies in animals vaccinated with rRCNV-G intradermally or intramuscularly suggested that the recombinant is effective and that RCNV would be a suitable substrate for further development of sheep vaccines. Poor antibody response to rRCNV-G given orally implied that it would be relatively harmless if inadvertently ingested by sheep. Virus transmission between vaccinated and sentinel sheep was not observed or detected serologically.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Viral / biosynthesis
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Antibodies, Viral / immunology
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Antigens, Viral*
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Cell Line
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Chlorocebus aethiops
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Cloning, Molecular
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Glycoproteins / genetics
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Glycoproteins / immunology*
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Neutralization Tests
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Poxviridae / genetics
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Poxviridae / immunology*
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Poxviridae / physiology
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Rabies Vaccines / adverse effects
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Rabies Vaccines / genetics
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Rabies Vaccines / immunology*
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Rabies virus / genetics
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Rabies virus / immunology
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Rabies virus / physiology
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Raccoons
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Sheep
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Vaccines, Synthetic / genetics
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Vaccines, Synthetic / immunology
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Viral Envelope Proteins / genetics
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Viral Envelope Proteins / immunology*
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Virus Replication
Substances
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Antibodies, Viral
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Antigens, Viral
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Glycoproteins
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Rabies Vaccines
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Vaccines, Synthetic
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Viral Envelope Proteins
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glycoprotein G, Rabies virus