[In vitro granulopoietic cultures. Analysis using monoclonal antibodies]

Sangre (Barc). 1993 Aug;38(4):331-4.
[Article in Spanish]

Abstract

Four monoclonal antibodies were applied, using an immunoenzymatic technique, the alkaline phosphatase anti-alkaline phosphatase, in granulopoietic cultures in vitro. In this manner, granulopoietic growth at tenth day of culture has been defined as positive for myeloid markers (CD15, CD11b) and negative for macrophagic (CD68) and proliferating cells (Ki 67) markers.

Publication types

  • English Abstract

MeSH terms

  • Adult
  • Aged
  • Antibodies, Monoclonal / immunology*
  • Antigens, CD / analysis
  • Biomarkers / analysis
  • Cell Differentiation
  • Cells, Cultured
  • Colony-Forming Units Assay
  • Female
  • Granulocytes*
  • Hematopoiesis
  • Hematopoietic Stem Cells / cytology*
  • Hematopoietic Stem Cells / immunology
  • Humans
  • Immunoenzyme Techniques
  • Ki-67 Antigen
  • Lymphoma / pathology
  • Macrophages*
  • Male
  • Middle Aged
  • Neoplasm Proteins / analysis
  • Nuclear Proteins / analysis

Substances

  • Antibodies, Monoclonal
  • Antigens, CD
  • Biomarkers
  • Ki-67 Antigen
  • Neoplasm Proteins
  • Nuclear Proteins