Monitoring the activity of antiviral therapy for HIV infection using a polymerase chain reaction method coupled with reverse transcription

AIDS. 1993 Nov:7 Suppl 2:S101-5. doi: 10.1097/00002030-199311002-00019.

Abstract

Aim: To monitor the anti-HIV-1 activity of antiretroviral agents in patients with HIV-1 infection.

Method: Quantification of viral RNA copy in plasma or serum using a polymerase chain reaction method coupled with reverse transcription.

Conclusions: The HIV-1 RNA copy number represents the HIV-1 viremia status in patients with HIV-1 infection. This copy number is likely to be useful in monitoring the effectiveness of antiviral therapy and the method is likely to be built into every clinical trial of anti-HIV-1 therapy in the near future.

Publication types

  • Review

MeSH terms

  • Antiviral Agents / therapeutic use*
  • Didanosine / therapeutic use
  • Drug Resistance, Microbial / genetics
  • HIV Core Protein p24 / blood
  • HIV Infections / drug therapy*
  • HIV Infections / microbiology*
  • HIV-1 / genetics*
  • HIV-1 / isolation & purification
  • Humans
  • Polymerase Chain Reaction / methods*
  • Polymerase Chain Reaction / statistics & numerical data
  • RNA, Viral / blood
  • RNA, Viral / genetics
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Transcription, Genetic
  • Viremia / drug therapy
  • Viremia / microbiology
  • Virus Replication

Substances

  • Antiviral Agents
  • HIV Core Protein p24
  • RNA, Viral
  • Didanosine