Effects of nitric oxide on red blood cells: changes in erythrocyte resistance to hypotonic hemolysis and potassium efflux by experimental maneuvers that decrease nitric oxide

Biochem Biophys Res Commun. 1994 Mar 15;199(2):447-54. doi: 10.1006/bbrc.1994.1249.

Abstract

To determine the role of nitric oxide (NO)-dependent mechanisms on erythrocyte properties, we exposed red cells to L-arginine competitive analogues, 8Br-cyclic guanosine monophosphate (8Br-cGMP) and neutrophil-eliminating filters. These treatments significantly decreased hypotonic hemolysis, increased potassium efflux and caused a spiculate change in erythrocyte morphology. These effects were related to a decrease of NO caused by the three types of treatments.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Animals
  • Arginine / analogs & derivatives*
  • Arginine / pharmacology*
  • Cyclic GMP / analogs & derivatives*
  • Cyclic GMP / pharmacology
  • Erythrocytes / drug effects
  • Erythrocytes / metabolism
  • Erythrocytes / physiology*
  • Female
  • Hemolysis* / drug effects
  • Humans
  • Hypotonic Solutions
  • Kinetics
  • Male
  • NG-Nitroarginine Methyl Ester
  • Neutrophils / physiology
  • Nitric Oxide / blood*
  • Potassium / blood*
  • Rats
  • Rats, Wistar
  • omega-N-Methylarginine

Substances

  • Hypotonic Solutions
  • omega-N-Methylarginine
  • 8-bromocyclic GMP
  • Nitric Oxide
  • Arginine
  • Cyclic GMP
  • Potassium
  • NG-Nitroarginine Methyl Ester