Noninvasive evaluation of cytochrome c oxidase activity of the liver. Its prognostic value for hepatic resection

Arch Surg. 1994 Mar;129(3):303-8. doi: 10.1001/archsurg.1994.01420270079018.

Abstract

Objective: To clarify the relationship between the noninvasive evaluation of hepatic mitochondrial function, the redox tolerance test, and cytochrome c oxidase activity of the liver, focusing on surgical risk in hepatic resection.

Design: Six-month randomized clinical trial.

Setting: Inpatients in surgical department.

Study participants: Forty patients who underwent hepatic resection (n = 36) and other abdominal operations (n = 4).

Intervention: Preoperative, noninvasive: The redox tolerance test, which measures the changes in arterial ketone body ratio in response to 75 g of oral glucose loading. Intraoperative, invasive: Cytochrome c oxidase activity and energy charge of the liver.

Main outcome measure: Correlation of the index in the redox tolerance test (RTI) with cytochrome c oxidase activity, both of which predict the postoperative course.

Results: The RTI values were negatively correlated with the maximal velocity (Vmax) and Michaelis constant (Km) values of cytochrome c oxidase activity. The maximal velocity and Michaelis constant values in patients with RTI values above or equal to 0.5 (group A, n = 29) were significantly lower than those in patients with RTI values below 0.5 (group B, n = 11). Eight (72.7%) of 11 patients in group B experienced postoperative complications.

Conclusion: The RTI is a noninvasive method of assessing the hepatic energy metabolism and can be a useful index for evaluating surgical risk in hepatectomy.

Publication types

  • Clinical Trial
  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenine Nucleotides / metabolism
  • Adult
  • Aged
  • Electron Transport Complex IV / metabolism*
  • Evaluation Studies as Topic
  • Female
  • Glucose Tolerance Test
  • Hepatectomy*
  • Humans
  • Ketone Bodies / blood
  • Liver / enzymology*
  • Male
  • Middle Aged
  • Mitochondria, Liver / enzymology
  • Oxidation-Reduction
  • Prognosis

Substances

  • Adenine Nucleotides
  • Ketone Bodies
  • Electron Transport Complex IV