Monoclonal non-specific suppressor factor (MNSF) inhibits the IL4 secretion by bone marrow-derived mast cell (BMMC)

FEBS Lett. 1994 Feb 21;339(3):239-42. doi: 10.1016/0014-5793(94)80423-0.

Abstract

We investigated whether monoclonal non-specific suppressor factor (MNSF) has an effect on the IL4 secretion by BMMC sensitized with anti-DNP IgE monoclonal antibody. The sensitized BMMC showed a maximum increase of IL4 secretion at 10 ng/ml antigen activation. When MNSF (10 U) was added, it remarkably suppressed the IL4 secretion (82 +/- 3%). MNSF also suppressed the IL4 secretion by the mast cell line, CFTL-15, but not CFTL-12. Northern blot analysis showed that, when the same amounts of MNSF were added, IL4 mRNA expression was decreased, suggesting that the suppression results from the inhibition of a transcript.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / pharmacology
  • Blotting, Northern
  • Bone Marrow Cells*
  • Dinitrophenols / immunology
  • Female
  • Immunoglobulin E / pharmacology
  • Interferon-gamma / pharmacology
  • Interleukin-4 / genetics
  • Interleukin-4 / metabolism*
  • Mast Cells / metabolism*
  • Mice
  • Mice, Inbred AKR
  • Mice, Inbred BALB C
  • RNA, Messenger / metabolism
  • Rats
  • Suppressor Factors, Immunologic / pharmacology*

Substances

  • Antibodies, Monoclonal
  • Dinitrophenols
  • RNA, Messenger
  • Suppressor Factors, Immunologic
  • monoclonal nonspecific suppressor factor
  • Interleukin-4
  • Immunoglobulin E
  • Interferon-gamma