The effect of a heterocyclic amine, 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1), on tyrosine hydroxylase mRNA in PC12h cells

Neurosci Lett. 1993 May 14;154(1-2):183-6. doi: 10.1016/0304-3940(93)90202-v.

Abstract

The effects of a naturally occurring heterocyclic amine, 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indol (Trp-P-1), on tyrosine hydroxylase (TH) mRNA levels were examined in PC12h cells using Northern blot analysis. Dose-dependency of Trp-P-1 for 24 h incubation gave biphasic results; 0.1 microM Trp-P-1 enhanced and 10 microM Trp-P-1 reduced TH mRNA levels. These effects were faded out in 48 h. One hundred microM Trp-P-1 caused the almost complete abolishment of TH mRNA expression as well as cell death. These results suggest the possibility that lower dose of Trp-P-1 might function as a trophic factor in contrast to neurotoxic effect in higher doses.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Northern
  • Carbolines / pharmacology*
  • Carbolines / toxicity
  • Cell Death / drug effects
  • Clone Cells
  • Cycloheximide / pharmacology
  • Dactinomycin / pharmacology
  • Mutagens / pharmacology*
  • Mutagens / toxicity
  • PC12 Cells
  • RNA, Messenger / biosynthesis*
  • Transcription, Genetic
  • Tyrosine 3-Monooxygenase / biosynthesis*

Substances

  • Carbolines
  • Mutagens
  • RNA, Messenger
  • Dactinomycin
  • 3-amino-1-methyl-5H-pyrido(4,3-b)indole
  • Cycloheximide
  • Tyrosine 3-Monooxygenase
  • 3-amino-1,4-dimethyl-5H-pyrido(4,3-b)indole