Adrenocortical ACTH receptors in pigs of differing in vivo response to adrenocorticotropin

Comp Biochem Physiol Comp Physiol. 1993 Jan;104(1):43-9. doi: 10.1016/0300-9629(93)90006-p.

Abstract

1. Adrenocortical membrane protein was isolated from the adrenal glands of 12 Large White x Landrace male pigs, six with high adrenocortical response to adrenocorticotropic hormone (ACTH) and six with low response. 2. The peptide (Phe2, Nle4) ACTH was iodinated by the chloramine-T method and served as the radioligand in receptor binding studies. 3. Only one class of ACTH receptor was detected, with Kd = 2.57 +/- 0.35 x 10(9) M and Bmax = 1.59 +/- 0.06 pmol/mg protein in high responders and Kd = 1.68 +/- 0.18 x 10(-9) M and Bmax = 1.17 +/- 0.11 pmol/mg protein in low responders. 4. The difference in the Bmax between high and low responders was significant (P < 0.05), the difference in Kd was not statistically significant.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adrenal Cortex / drug effects
  • Adrenal Cortex / metabolism*
  • Adrenocorticotropic Hormone / administration & dosage*
  • Adrenocorticotropic Hormone / analogs & derivatives
  • Adrenocorticotropic Hormone / pharmacokinetics
  • Animals
  • Cell Membrane / drug effects
  • Cell Membrane / metabolism
  • Cosyntropin / analogs & derivatives
  • Cosyntropin / metabolism
  • Dose-Response Relationship, Drug
  • Kidney / drug effects
  • Kidney / metabolism
  • Liver / drug effects
  • Liver / metabolism
  • Male
  • Membrane Proteins / pharmacology
  • Protein Binding / drug effects
  • Receptors, Corticotropin
  • Receptors, Pituitary Hormone / drug effects*
  • Receptors, Pituitary Hormone / metabolism*
  • Swine
  • Time Factors

Substances

  • Membrane Proteins
  • Receptors, Corticotropin
  • Receptors, Pituitary Hormone
  • Cosyntropin
  • Adrenocorticotropic Hormone
  • ACTH (1-24), Phe(2)-Nle(4)-