Abstract
A cDNA clone encoding a psychrophilic cod chymotrypsinogen has been isolated and characterized. The predicted amino acid sequence reveals a preproenzyme of 263 amino acids containing a unique 18 residue signal sequence. Amino acid sequence identity between the cod and mammalian chymotrypsinogens is 64-68%. Two highly conserved proline residues are substituted in cod chymotrypsin.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Atlantic Ocean
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Base Sequence
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Chymotrypsinogen / classification
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Chymotrypsinogen / genetics*
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Cloning, Molecular
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Cold Temperature
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DNA, Complementary / genetics
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Enzyme Precursors / genetics*
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Fishes / genetics*
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Marine Biology
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Molecular Sequence Data
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
Substances
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DNA, Complementary
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Enzyme Precursors
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prechymotrypsinogen 2
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Chymotrypsinogen